Possible role of rat fatty acid-binding proteins in the intestine as carriers of phenol and phthalate derivatives

Biochem Biophys Res Commun. 1990 May 16;168(3):1053-8. doi: 10.1016/0006-291x(90)91136-g.

Abstract

Fatty acid-binding proteins of hepatic and intestinal type and gastrotropin-like protein (GTLP) were purified from rat intestinal cytosol by Sephadex G-75 gel filtration and DEAE-cellulose, CM-cellulose, and hydroxylapatite chromatographies. In addition to fatty acids, butylated hydroxytoluene (BHT), phthalate dibutyl, and di(2-ethylhexyl) esters (DBP and DEHP) were identified by gas liquid chromatography and mass spectrometry as endogenous ligands from the extract of either fatty acid-binding protein superfamily. These protein families in the intestine may have an important role as carriers in the initial step of arresting these exogenous pollutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins* / isolation & purification
  • Carrier Proteins* / metabolism
  • Carrier Proteins* / physiology*
  • Chromatography, Gas
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Gas Chromatography-Mass Spectrometry
  • Gastrointestinal Hormones / isolation & purification
  • Gastrointestinal Hormones / physiology
  • Intestinal Mucosa / metabolism*
  • Ligands
  • Liver / metabolism
  • Male
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Phenols / metabolism*
  • Phthalic Acids / metabolism*
  • Rats

Substances

  • Carrier Proteins
  • Fabp7 protein, rat
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Gastrointestinal Hormones
  • Ligands
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Phenols
  • Phthalic Acids
  • fatty acid-binding protein 6