Abstract
The human liver-derived cell line, Hep G2, has aryl hydrocarbon hydroxylase and 7-ethoxycoumarin o-de-ethylase activities. Partial purification of cytochrome P-450 from Hep G2 cells provided spectral evidence of this hemeprotein in the purified fraction. These results suggest that Hep G2 cells will be useful for the study of cytochrome P-450 and the regulation of mixed function oxidase activities in liver cells of human origin.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
7-Alkoxycoumarin O-Dealkylase
-
Aryl Hydrocarbon Hydroxylases / metabolism*
-
Carcinoma, Hepatocellular / metabolism*
-
Cell Line
-
Cytochrome P-450 Enzyme System / isolation & purification
-
Cytochrome P-450 Enzyme System / metabolism*
-
Humans
-
Kinetics
-
Liver Neoplasms / metabolism*
-
Microsomes / metabolism
-
Oxygenases / metabolism*
Substances
-
Cytochrome P-450 Enzyme System
-
Oxygenases
-
7-Alkoxycoumarin O-Dealkylase
-
Aryl Hydrocarbon Hydroxylases