Abstract
The activities of testosterone hydroxylases and erythromycin N-demethylase were significantly higher in liver microsomes from female hamsters than in the male counterparts. SDS-polyacrylamide gel electrophoresis revealed a difference in protein composition between male and female liver microsomes in the molecular mass region comprising cytochrome P-450. Western blot analysis showed further that antibodies to rat male-specific cytochrome P-450 crossreacted with at least two proteins in both male and female hamster microsomes, but one of the female proteins had a different molecular mass from that of the male proteins. It is concluded that sex difference in liver microsomal cytochrome P-450 is not restricted to rats and mice, as has previously been believed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aryl Hydrocarbon Hydroxylases*
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Cricetinae
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Cytochrome P-450 CYP3A
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Cytochrome P-450 Enzyme System / isolation & purification
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Cytochrome P-450 Enzyme System / metabolism
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Cytochrome b Group / isolation & purification
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Cytochrome b Group / metabolism
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Cytochromes b5
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Erythromycin / metabolism*
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Female
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Male
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Mesocricetus
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Microsomes, Liver / enzymology*
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Oxidation-Reduction
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Oxidoreductases, N-Demethylating / metabolism*
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Sex Factors
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Steroid Hydroxylases / metabolism*
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Testosterone / metabolism*
Substances
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Cytochrome b Group
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Testosterone
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Erythromycin
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Cytochromes b5
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Cytochrome P-450 Enzyme System
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Steroid Hydroxylases
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Aryl Hydrocarbon Hydroxylases
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Cytochrome P-450 CYP3A
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testosterone 7-alpha-hydroxylase, hamster
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Oxidoreductases, N-Demethylating