25-Hydroxyvitamin D3 3-sulphate is a major circulating form of vitamin D in man

FEBS Lett. 1985 Oct 28;191(2):171-5. doi: 10.1016/0014-5793(85)80002-8.

Abstract

25-Hydroxyvitamin D3 3 beta-sulphate has been identified in human plasma. The compound was isolated by anion-exchange chromatography and following hydrolysis it was characterized by high-performance liquid chromatography and gas chromatography-mass spectrometry. The mean concentration of sulphated 25-hydroxyvitamin D3 in plasma from 60 patients was 16.7 +/- 7.1 ng/ml and the levels often exceeded those of the corresponding free compound. The study also shows that unconjugated 25-hydroxyvitamin D3 is not readily sulphated by man in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcifediol / analogs & derivatives*
  • Calcifediol / analysis
  • Calcifediol / blood*
  • Cholesterol / metabolism
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Lactation
  • Liver / metabolism
  • Milk, Human / analysis
  • Pregnancy
  • Reference Values
  • Sulfates / blood
  • Sulfates / metabolism

Substances

  • Sulfates
  • Cholesterol
  • 25-hydroxyvitamin D3 3-sulfate ester
  • Calcifediol