Abstract
Four metabolites of nicotine, including two glucuronides, have been separated by high-performance liquid chromatography. This separation was applied to identification of biliary metabolites of radiolabeled nicotine by radiometric detection. S-(-)-Nicotine N-glucuronide was synthesized and used as a standard in method development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bile / chemistry*
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Chromatography, High Pressure Liquid / methods*
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Glucuronates / chemical synthesis*
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Magnetic Resonance Spectroscopy
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Male
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Mass Spectrometry
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Nicotine / analogs & derivatives*
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Nicotine / analysis*
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Nicotine / chemical synthesis
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Nicotine / metabolism
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Radiometry
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Rats
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Rats, Sprague-Dawley
Substances
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Glucuronates
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nicotine N-glucuronide
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Nicotine