Identification of radiolabeled metabolites of nicotine in rat bile. Synthesis of S-(-)-nicotine N-glucuronide and direct separation of nicotine-derived conjugates using high-performance liquid chromatography

M J Seaton; E S Vesell; H Luo; E M Hawes

Identification of radiolabeled metabolites of nicotine in rat bile. Synthesis of S-(-)-nicotine N-glucuronide and direct separation of nicotine-derived conjugates using high-performance liquid chromatography

J Chromatogr. 1993 Nov 17;621(1):49-53.

Authors

M J Seaton¹, E S Vesell, H Luo, E M Hawes

Affiliation

¹ Department of Pharmacology, Pennsylvania State University, College of Medicine, Hershey 17033.

PMID: 8308087

Abstract

Four metabolites of nicotine, including two glucuronides, have been separated by high-performance liquid chromatography. This separation was applied to identification of biliary metabolites of radiolabeled nicotine by radiometric detection. S-(-)-Nicotine N-glucuronide was synthesized and used as a standard in method development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bile / chemistry*
Chromatography, High Pressure Liquid / methods*
Glucuronates / chemical synthesis*
Magnetic Resonance Spectroscopy
Male
Mass Spectrometry
Nicotine / analogs & derivatives*
Nicotine / analysis*
Nicotine / chemical synthesis
Nicotine / metabolism
Radiometry
Rats
Rats, Sprague-Dawley

Substances

Glucuronates
nicotine N-glucuronide
Nicotine