Abstract
Effects of T3 treatment (day 2-day 12) on the expression of the insulin-regulated GLUT4, on 2-deoxyglucose uptake, and on glycogen synthesis were studied in ARC after 12 days of culture in T3-depleted 20% fetal calf serum. GLUT4 mRNA expression was low in controls, but increased in a dose-dependent manner by T3 treatment up to 2.8-fold at 100 nM. In parallel, 100 nM T3 increased basal 2-deoxyglucose uptake 1.95-fold and insulin-stimulated uptake 1.75-fold. In addition, T3 enhanced basal and insulin-stimulated [14C] glucose incorporation into glycogen 1.86- and 1.5-fold, respectively. Hence, ARC may meet part of their increased energy requirements in response to T3 by enhancing GLUT4 expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Biological Transport
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Cells, Cultured
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DNA Primers
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Deoxyglucose / metabolism*
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Female
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Glucose Transporter Type 4
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Glycogen / biosynthesis*
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Glycogen / metabolism
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Heart / drug effects
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Insulin / pharmacology
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Molecular Sequence Data
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Monosaccharide Transport Proteins / genetics*
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Monosaccharide Transport Proteins / metabolism
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Muscle Proteins*
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Myocardium / cytology
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Myocardium / metabolism*
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Triiodothyronine / pharmacology*
Substances
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DNA Primers
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Glucose Transporter Type 4
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Insulin
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Monosaccharide Transport Proteins
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Muscle Proteins
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RNA, Messenger
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Slc2a4 protein, rat
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Triiodothyronine
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Glycogen
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Deoxyglucose