Abstract
In the present study, a rat liver cytosolic enzyme responsible for reduction of S-(-)-nicotine-1'-N-oxide to S-(-)-nicotine was investigated. We found that aldehyde oxidase (EC 1.2.3.1) can function as a S-(-)-nicotine-1'-N-oxide reductase in the presence of its electron donor such as 2-hydroxypyrimidine. The apparent K(m) and Vmax values of the enzyme for the N-oxide were 0.24 mM and 30.3 nmol/10 min/mg protein, respectively.
MeSH terms
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Aldehyde Oxidase
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Aldehyde Oxidoreductases / antagonists & inhibitors
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Aldehyde Oxidoreductases / metabolism*
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Animals
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Benzaldehydes / pharmacology
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Cyclic N-Oxides / metabolism
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Cytosol / enzymology
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Enzyme Inhibitors / pharmacology
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Kinetics
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Liver / drug effects
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Liver / enzymology*
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Male
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Nicotine / analogs & derivatives
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Nicotine / metabolism*
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Pyrimidines / pharmacology
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Rats
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Rats, Inbred Strains
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Rats, Sprague-Dawley
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Rats, Wistar
Substances
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Benzaldehydes
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Cyclic N-Oxides
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Enzyme Inhibitors
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Pyrimidines
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2-hydroxypyrimidine
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nicotine 1-N-oxide
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Nicotine
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Aldehyde Oxidoreductases
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Aldehyde Oxidase
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benzaldehyde