Abstract
Various human sulfotransferases (hP-PST, hM-PST, hHST) and rat sulfotransferases (rPST-IV, rHSTa) have already been expressed in Ames' Salmonella strains (in particular in TA1538). Now a further strain, TA1538-hEST, which expresses the human estrogen sulfotransferase (hEST), has been constructed. This strain activated the primary benzylic alcohol 1-hydroxymethylpyrene (1-HMP) and the secondary benzylic alcohol 1-hydroxyethylpyrene (1-HEP) to mutagens. Human sulfotransferases hEST and hHST both activated 1-HEP, but they differed substantially in their enantioselectivity for this compound.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzyl Alcohols / pharmacokinetics
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Benzyl Alcohols / toxicity
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Blotting, Western
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Cytosol / enzymology
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Humans
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Mutagenicity Tests
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Mutagens / pharmacokinetics*
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Mutagens / toxicity
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Rats
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Salmonella typhimurium / drug effects
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Salmonella typhimurium / enzymology*
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Salmonella typhimurium / genetics
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Stereoisomerism
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Substrate Specificity
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Sulfotransferases / biosynthesis
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Sulfotransferases / genetics
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Sulfotransferases / metabolism*
Substances
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Benzyl Alcohols
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Mutagens
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Sulfotransferases
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alcohol sulfotransferase
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estrone sulfotransferase