Abstract
26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 (ST-630) is a newly developed agent to maintain the levels of calcium and phosphorus in blood. Herein, we investigated the effect of this compound on the expression of vitamin-D-responsive genes in vitamin-D-deficient mice. ST-630 was more effective than 1, 25-dihydroxyvitamin D3 [1,25(OH)2D3] with respect to the induction of Cyp24 and calbindin-D9k mRNAs in the kidney and in the small intestine. Moreover, the increase in mRNA levels of vitamin-D-responsive genes induced by ST-630 lasted longer than that induced by 1,25(OH)2D3. These results indicate that ST-630 was more effective in inducing Cyp24 and calbindin-D9k gene expression than 1, 25(OH)2D3 when both compounds were injected into vitamin-D-deficient mice.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calbindins
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Calcitriol / analogs & derivatives*
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Calcitriol / pharmacology
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Calcitriol / therapeutic use*
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Calcium / blood
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Cytochrome P-450 Enzyme System / biosynthesis
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Enzyme Induction / drug effects
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Gene Expression Regulation / drug effects
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Injections, Intravenous
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Intestine, Small / drug effects*
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Intestine, Small / enzymology
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Kidney / drug effects*
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Kidney / metabolism
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Male
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Mice
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Mice, Inbred ICR
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RNA, Messenger / genetics
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S100 Calcium Binding Protein G / drug effects
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S100 Calcium Binding Protein G / genetics
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Steroid Hydroxylases / biosynthesis
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Vitamin D / genetics*
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Vitamin D / metabolism
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Vitamin D Deficiency / blood
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Vitamin D Deficiency / drug therapy*
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Vitamin D3 24-Hydroxylase
Substances
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Calbindins
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RNA, Messenger
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S100 Calcium Binding Protein G
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S100g protein, mouse
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Vitamin D
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Cytochrome P-450 Enzyme System
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Steroid Hydroxylases
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Vitamin D3 24-Hydroxylase
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Calcitriol
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26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3
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Calcium