User profiles for Bhagwat Prasad
Bhagwat PrasadAssociate Professor, Pharmaceutical Sci, Director, PRINCE, Washington State University … Verified email at wsu.edu Cited by 5297 |
Metabolite identification by liquid chromatography-mass spectrometry
Metabolite identification (Met ID) is important during the early stages of drug discovery and
development, as the metabolic products may be pharmacologically active or toxic in nature. …
development, as the metabolic products may be pharmacologically active or toxic in nature. …
Protein abundance of clinically relevant multidrug transporters along the entire length of the human intestine
Intestinal transporters are crucial determinants in the oral absorption of many drugs. We
therefore studied the mRNA expression (N = 33) and absolute protein content (N = 10) of …
therefore studied the mRNA expression (N = 33) and absolute protein content (N = 10) of …
Interindividual variability in cytochrome P450–mediated drug metabolism
TS Tracy, AS Chaudhry, B Prasad, KE Thummel… - Drug Metabolism and …, 2016 - ASPET
The cytochrome P450 (P450) enzymes are the predominant enzyme system involved in human
drug metabolism. Alterations in the expression and/or activity of these enzymes result in …
drug metabolism. Alterations in the expression and/or activity of these enzymes result in …
Quantitative proteomics in translational absorption, distribution, metabolism, and excretion and precision medicine
A reliable translation of in vitro and preclinical data on drug absorption, distribution, metabolism,
and excretion (ADME) to humans is important for safe and effective drug development. …
and excretion (ADME) to humans is important for safe and effective drug development. …
Interspecies variability in expression of hepatobiliary transporters across human, dog, monkey, and rat as determined by quantitative proteomics
L Wang, B Prasad, L Salphati, X Chu, A Gupta… - Drug Metabolism and …, 2015 - ASPET
We quantified, by liquid chromatography tandem mass spectrometry, transporter protein
expression of BSEP, MATE1, MRP3, MRP4, NTCP, and OCT1 in our human liver bank (n = 55) …
expression of BSEP, MATE1, MRP3, MRP4, NTCP, and OCT1 in our human liver bank (n = 55) …
Interindividual variability in hepatic organic anion-transporting polypeptides and P-glycoprotein (ABCB1) protein expression: quantification by liquid chromatography …
Interindividual variability in protein expression of organic anion-transporting polypeptides (OATPs)
OATP1B1, OATP1B3, OATP2B1, and multidrug resistance-linked P-glycoprotein (P-gp…
OATP1B1, OATP1B3, OATP2B1, and multidrug resistance-linked P-glycoprotein (P-gp…
Abundance of drug transporters in the human kidney cortex as quantified by quantitative targeted proteomics
Protein expression of renal uptake and efflux transporters was quantified by quantitative
targeted proteomics using the surrogate peptide approach. Renal uptake transporters assessed …
targeted proteomics using the surrogate peptide approach. Renal uptake transporters assessed …
Targeted LC-MS/MS proteomics-based strategy to characterize in vitro models used in drug metabolism and transport studies
Subcellular fractionation of tissue homogenate provides enriched in vitro models (eg,
microsomes, cytosol, or membranes), which are routinely used in the drug metabolism or …
microsomes, cytosol, or membranes), which are routinely used in the drug metabolism or …
Strategies for characterizing sildenafil, vardenafil, tadalafil and their analogues in herbal dietary supplements, and detecting counterfeit products containing these …
The success of synthetic phosphodiesterase type-5 (PDE-5)-inhibitor drugs (viz sildenafil,
vardenafil and tadalafil), which are constituents of popular brands (viz Viagra, Levitra and …
vardenafil and tadalafil), which are constituents of popular brands (viz Viagra, Levitra and …
Ontogeny of hepatic drug transporters as quantified by LC‐MS/MS proteomics
B Prasad, A Gaedigk, M Vrana… - Clinical …, 2016 - Wiley Online Library
Protein expression of major hepatic uptake and efflux drug transporters in human pediatric (n
= 69) and adult (n = 41) livers was quantified by liquid chromatography / tandem mass …
= 69) and adult (n = 41) livers was quantified by liquid chromatography / tandem mass …