User profiles for Bhagwat Prasad

Bhagwat Prasad

Associate Professor, Pharmaceutical Sci, Director, PRINCE, Washington State University …
Verified email at wsu.edu
Cited by 5297

Metabolite identification by liquid chromatography-mass spectrometry

B Prasad, A Garg, H Takwani, S Singh - TrAC Trends in Analytical …, 2011 - Elsevier
Metabolite identification (Met ID) is important during the early stages of drug discovery and
development, as the metabolic products may be pharmacologically active or toxic in nature. …

Protein abundance of clinically relevant multidrug transporters along the entire length of the human intestine

…, J Lapczuk, M Ostrowski, Y Lai, B Prasad… - Molecular …, 2014 - ACS Publications
Intestinal transporters are crucial determinants in the oral absorption of many drugs. We
therefore studied the mRNA expression (N = 33) and absolute protein content (N = 10) of …

Interindividual variability in cytochrome P450–mediated drug metabolism

TS Tracy, AS Chaudhry, B Prasad, KE Thummel… - Drug Metabolism and …, 2016 - ASPET
The cytochrome P450 (P450) enzymes are the predominant enzyme system involved in human
drug metabolism. Alterations in the expression and/or activity of these enzymes result in …

Quantitative proteomics in translational absorption, distribution, metabolism, and excretion and precision medicine

…, L Kruger, S Sharma, VS Mettu, A Basit, B Prasad - Pharmacological …, 2022 - ASPET
A reliable translation of in vitro and preclinical data on drug absorption, distribution, metabolism,
and excretion (ADME) to humans is important for safe and effective drug development. …

Interspecies variability in expression of hepatobiliary transporters across human, dog, monkey, and rat as determined by quantitative proteomics

L Wang, B Prasad, L Salphati, X Chu, A Gupta… - Drug Metabolism and …, 2015 - ASPET
We quantified, by liquid chromatography tandem mass spectrometry, transporter protein
expression of BSEP, MATE1, MRP3, MRP4, NTCP, and OCT1 in our human liver bank (n = 55) …

Interindividual variability in hepatic organic anion-transporting polypeptides and P-glycoprotein (ABCB1) protein expression: quantification by liquid chromatography …

B Prasad, R Evers, A Gupta, CECA Hop… - Drug Metabolism and …, 2014 - ASPET
Interindividual variability in protein expression of organic anion-transporting polypeptides (OATPs)
OATP1B1, OATP1B3, OATP2B1, and multidrug resistance-linked P-glycoprotein (P-gp…

Abundance of drug transporters in the human kidney cortex as quantified by quantitative targeted proteomics

B Prasad, K Johnson, S Billington, C Lee… - Drug Metabolism and …, 2016 - ASPET
Protein expression of renal uptake and efflux transporters was quantified by quantitative
targeted proteomics using the surrogate peptide approach. Renal uptake transporters assessed …

Targeted LC-MS/MS proteomics-based strategy to characterize in vitro models used in drug metabolism and transport studies

…, S Heyward, JD Unadkat, B Prasad - Analytical …, 2018 - ACS Publications
Subcellular fractionation of tissue homogenate provides enriched in vitro models (eg,
microsomes, cytosol, or membranes), which are routinely used in the drug metabolism or …

Strategies for characterizing sildenafil, vardenafil, tadalafil and their analogues in herbal dietary supplements, and detecting counterfeit products containing these …

S Singh, B Prasad, AA Savaliya, RP Shah… - TrAC Trends in …, 2009 - Elsevier
The success of synthetic phosphodiesterase type-5 (PDE-5)-inhibitor drugs (viz sildenafil,
vardenafil and tadalafil), which are constituents of popular brands (viz Viagra, Levitra and …

Ontogeny of hepatic drug transporters as quantified by LC‐MS/MS proteomics

B Prasad, A Gaedigk, M Vrana… - Clinical …, 2016 - Wiley Online Library
Protein expression of major hepatic uptake and efflux drug transporters in human pediatric (n
= 69) and adult (n = 41) livers was quantified by liquid chromatography / tandem mass …