Chemical inhibition is the widely used method in reaction phenotyping assays for estimation
of specific enzyme contribution to a given metabolic pathway. The results from phenotyping …

Measurement of unbound test compound concentrations at the biophase is routinely carried
out in the drug discovery. Microdialysis is an established sampling technique for in vivo …

The objective of the study was to determine the effect of fatty acids on CYP enzymes and the
effect of BSA on intrinsic clearance of probe substrates. The inhibitory effect of thirteen fatty …

A sensitive LC–MS/MS method was developed and validated for the quantification of almotriptan
in rat brain and blood dialysates. Almotriptan is a 5HT 1B/1D receptor agonist used for …

A simple and rapid LC–MS/MS method was developed and validated for the quantification of
epalrestat, an aldose reductase inhibitor for the treatment of diabetic neuropathy. Following …

Objectives The objective of the current investigation was to develop a simple, rapid method
for determining in-vivo 5-hydroxytryptamine type 4 receptor (5-HT 4 R) occupancy in rat brain …

Deferasirox is an iron chelating agent for the treatment of transfusional iron over load in
patients with chronic anemia. These anemic patients require close monitoring of the deferasirox …

The objective is to evaluate methoxsalen as an in vitro phenotyping tool in comparison to
ABT as a nonspecific inactivator of P450 mediated metabolism. The reversible inhibition of …

Background: Skin is the target site to evaluate the pharmacokinetic parameters of topical
applications. Sample preparation is one of the influential steps in the bioanalysis of drugs in the …

A simple and sensitive LC‐MS/MS method was developed and validated for the quantitation
of pitolisant, an H 3 receptor antagonist/inverse agonist. Acetonitrile protein precipitation …