Development of a human lymphoblastoid cell line constitutively expressing human CYP1B1 cDNA: substrate specificity with model substrates and promutagens

CL Crespi, B W-Penman, DT Steimel, T Smith… - …, 1997 - academic.oup.com
An AHH-1 TK +/− cell derivative was developed that stably expresses human cytochrome
P4501B1 (CYP1B1) cDNA in an extrachromosomal vector which confers resistance to 1-…

The development of a human cell line stably expressing human CYP3A4: role in the metabolic activation of aflatoxin B1 and comparison to CYP1A2 and CYP2A3

CL Crespi, BW Penman, DT Steimel… - …, 1991 - academic.oup.com
We have developed a human lymphoblastoid cell line, designated 3A4/HoI, which stably
expresses human CYP3A4 cDNA. This cell line exhibited testosterone 6β-hydroxylase activity, …

A metabolically competent human cell line expressing five cDNAs encoding procarcinogen-activating enzymes: application to mutagenicity testing

CL Crespi, FJ Gonzalez, DT Steimel… - Chemical research in …, 1991 - ACS Publications
A human B-lymphoblastoid cell line, designated MCL-5, constitutively expressing human
cytochrome P-450 CYP1A1 and also expressing five transfected human cDNAs encoding drug-…

Detection and characterization of novel polymorphisms in the CYP2E1 gene

…, J Grove, I de Waziers, DT Steimel… - Pharmacogenetics …, 1998 - journals.lww.com
To investigate whether interindividual variation in CYP2E1 levels can be explained by
genetic polymorphism, we analysed DNA samples from 40 healthy individuals by single-strand …

Human cytochrome P450IIA3: cDNA sequence role of the enzyme in the metabolic of promutagens comparison to nitrosamine activation by human cytochrome …

…, A Stark, A Parkinson, T Turner, DT Steimel… - …, 1990 - academic.oup.com
We report that, in a human cell line, human cytochrome P450IIA3 is capable of metabolizing
aflatoxin B 1 benzo[a]pyrene, N-nitrosodimethylamine (NDMA) and N-nitroso diethylamine (…

Characterization of cytochrome P450 2D6. 1 (CYP2D6. 1), CYP2D6. 2, and CYP2D6. 17 activities toward model CYP2D6 substrates dextromethorphan, bufuralol, and …

KA Marcucci, RE Pearce, C Crespi, DT Steimel… - Drug Metabolism and …, 2002 - ASPET
Over 50 allelic variants of cytochrome P450 2D6 (CYP2D6) encoding fully functional,
reduced-activity, or nonfunctional proteins have been described. Compared with Caucasians, …

Stable expression of human cytochrome P450IA2 cDNA in a human lymphoblastoid cell line: Role of the enzyme in the metabolic activation of aflatoxin B1

CL Crespi, DT Steimel, T Aoyama… - Molecular …, 1990 - Wiley Online Library
A human lymphoblastoid cell line stably expressing a human cytochrome P4501A2 cDNA
was developed. This recombinant cell line displayed P4501A2 protein and estradiol2‐…

Analysis of drug transport and metabolism in cell monolayer systems that have been modified by cytochrome P4503A4 cDNA-expression

…, L Fox, P Stocker, M Hu, DT Steimel - European journal of …, 2000 - Elsevier
Human CYP3A4, the major human, intestinal, drug metabolizing cytochrome P450, has
been introduced into three mammalian cell lines (Caco-2, MDCK and LLC-PK 1 ) suitable for …

Comparison of substrate metabolism by wild type CYP2D6 protein and a variant containing methionine, not valine, at position 374

CL Crespi, DT Steimel, BW Penman… - Pharmacogenetics …, 1995 - journals.lww.com
We have analysed kinetic parameters of cDNA-derived CYP2D6 proteins derived from the
original CYP2D6 cDNA isolate (Gonzalez FJ et al. Nature 1988: 331, 442-446) which contains …

Skills-based volunteering as both work and not work: A tension-centered examination of constructions of “volunteer”

S Steimel - VOLUNTAS: International Journal of Voluntary and …, 2018 - Springer
… She then explained, “I think if you don’t make it, I’ve heard at least that if you don’t do the
hours that you’re asked to do, you get an email from the CEO of the firm at the end of the year.” …