Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range

…, S Stein, P Boehlen, W Dairman, W Leimgruber… - Science, 1972 - science.org
Fluorescamine is a new reagent for the detection of primary amines in the picomole range.
Its reaction with amines is almost instantaneous at room temperature in aqueous media. The …

Fluorometric assay of proteins in the nanogram range

P Böhlen, S Stein, W Dairman… - Archives of …, 1973 - articles.researchsolutions.com
The reagent fluorescamine has been used for the fluorometric assay of proteins based on
their content of free amino groups and its applications to the monitoring of proteins during …

Preparation and properties of a homogeneous aromatic L-amino acid decarboxylase from hog kidney

JG Christenson, W Dairman, S Udenfriend - Archives of biochemistry and …, 1970 - Elsevier
… Control tubes containing 0.05 bCi of 3-Wserotonin (creatinine sulfate complex, … containing
0.5 pCi of 1-W-phenylalanine. … study (see footnote 1) W-CO2 was evolved from uniformly …

On the identity of DOPA decarboxylase and 5-hydroxytryptophan decarboxylase

JG Christenson, W Dairman… - Proceedings of the …, 1972 - National Acad Sciences
Simultaneous immunological titration of DOPA and 5-hydroxytryptophan decarboxylase
activities, from a number of tissues of various species, showed that the two activities were not …

Condensation of ninhydrin with aldehydes and primary amines to yield highly fluorescent ternary products: I. Studies on the Mechanism of the Reaction and Some …

K Samejima, W Dairman, S Udenfriend - Analytical biochemistry, 1971 - Elsevier
… 1 It0 ni:w .qwctromctcr. The procedure of Wang et al. (11) for I~hcnylnlaninc assay is
carriccl out) in the following manner: A mixture of 20 pl of l~hcngl:~lnnine (12 m31), 37.5 pl of …

Changes in tyrosine hydroxylase and dopa decarboxylase induced by pharmacological agents

W Dairman, JG Christenson, S Udenfriend - Pharmacological Reviews, 1972 - ASPET
The administration of L-dopa for several days has been shown to result in decreased activity
in vitro of the catecholamine biosynthetic enzymes in peripheral tissues, but not in the brain. …

Condensation of ninhydrin with aldehydes and primary amines to yield highly fluorescent ternary products: II. Application to the Detection and Assay of Peptides …

K Samejima, W Dairman, J Stone, S Udenfriend - Analytical Biochemistry, 1971 - Elsevier
… This report concerns applications of the waction to the quantitative assay of lwpiides, amino
acids, and aminea in solution and for detection ou paper and thin-layer chromatograms. …

Decrease in liver aromatic L-amino-acid decarboxylase produced by chronic administration of L-dopa

W Dairman, JG Christenson… - Proceedings of the …, 1971 - National Acad Sciences
L-Dopa, an intermediate in the biosynthesis of catecholamines, is now widely used in the
treatment of parkinsonism. A number of clinical observations suggested that L-dopa may …

Decarboxylation to tyramine: a major route of tyrosine metabolism in mammals

JC David, W Dairman… - Proceedings of the …, 1974 - National Acad Sciences
Metabolism of tyrosine was examined in mice, some of which had been treated with an
inhibitor of aromatic-L-amino-acid decarboxylase. The results of the study indicate that as the …

Increased synthesis of catecholamines in the intact rat following administration of α-adrenergic blocking agents

W DAIRMAN, R GORDON, S SPECTOR… - Molecular …, 1968 - ASPET
The present study was undertaken to evaluate the effects of α-blocking agents on the rate of
catecholamine synthesis in vivo. It was found that phentolamine and phenoxybenzamine …