Approach to the comprehensive analysis of glycoproteins isolated from human serum using a multi-lectin affinity column

Z Yang, WS Hancock - Journal of chromatography A, 2004 - Elsevier
Glycosylation is one the most common post-translational modifications (PTM) and glycoproteins
play fundamental roles in a diversity of biological processes. The development of an …

Recommendations for validation of LC-MS/MS bioanalytical methods for protein biotherapeutics

…, F Garofolo, S Kaur, GA Schultz, K Xu, Z Yang… - The AAPS journal, 2015 - Springer
This paper represents the consensus views of a cross-section of companies and
organizations from the USA and Canada regarding the validation and application of liquid …

MDR1 haplotypes significantly minimize intracellular uptake and transcellular P‐gp substrate transport in recombinant LLC‐PK1 cells

NN Salama, Z Yang, T Bui, RJY Ho - Journal of pharmaceutical sciences, 2006 - Elsevier
To date, research on the effect of single nucleotide polymorphisms (SNPs) on P‐glycoprotein
(P‐gp) expression and functionality has rendered inconsistent results. This study …

LC− MS/MS Approach for Quantification of Therapeutic Proteins in Plasma Using a Protein Internal Standard and 2D-Solid-Phase Extraction Cleanup

Z Yang, M Hayes, X Fang, MP Daley… - Analytical …, 2007 - ACS Publications
The bioanalysis of plasma samples generated from in vivo studies of therapeutic proteins is
of increasing interesting in the biopharmaceutical industry. The conventional ELISA …

Multilectin affinity chromatography for characterization of multiple glycoprotein biomarker candidates in serum from breast cancer patients

Z Yang, LE Harris, DE Palmer-Toy… - Clinical …, 2006 - academic.oup.com
Background: Glycoproteins are often associated with cancer and are important in serum
studies, for which glycosylation is a common posttranslational modification. Methods: We used …

Monitoring glycosylation pattern changes of glycoproteins using multi-lectin affinity chromatography

Z Yang, WS Hancock - Journal of Chromatography A, 2005 - Elsevier
Previously, we reported that the distribution of glycoproteins into the lectin displacement
fractions of a multi-lectin affinity column was determined by the glycosylation patterns of the …

Vulnerability of small-cell lung cancer to apoptosis induced by the combination of BET bromodomain proteins and BCL2 inhibitors

LT Lam, X Lin, EJ Faivre, Z Yang, X Huang… - Molecular cancer …, 2017 - AACR
Ten percent to 15% of all lung cancers are small-cell lung cancer (SCLC). SCLC usually
grows and metastasizes before it is diagnosed and relapses rapidly upon treatment. …

Deformable ferrofluid-based millirobot with high motion accuracy and high output force

D Chen, Z Yang, Y Ji, Y Dai, L Feng, F Arai - Applied Physics Letters, 2021 - pubs.aip.org
The magnetically actuated robot has valuable potential in biomedicine, bioengineering, and
biophysics for its capability to precisely manipulate particles or biological tissues. However, …

Targeting lineage-specific MITF pathway in human melanoma cell lines by A-485, the selective small-molecule inhibitor of p300/CBP

R Wang, Y He, V Robinson, Z Yang, P Hessler… - Molecular cancer …, 2018 - AACR
Metastatic melanoma is responsible for approximately 80% of deaths from skin cancer.
Microphthalmia-associated transcription factor (MITF) is a melanocyte-specific transcription factor …

A study of glycoproteins in human serum and plasma reference standards (HUPO) using multilectin affinity chromatography coupled with RPLC‐MS/MS

Z Yang, WS Hancock, TR Chew, L Bonilla - Proteomics, 2005 - Wiley Online Library
The glycoproteome is a major subproteome present in human plasma. In this study, we isolated
and characterized approximately 150 glycoproteins from the human plasma and serum …