The Effects of Endoxifen and Other Major Metabolites of Tamoxifen on the Sulfation of Estradiol Catalyzed by Human Cytosolic Sulfotransferases hSULT1E1 and hSULT1A1*1

Data Supplement

Files in this Data Supplement:

• Supplemental Figures -

  Supplemental Figure 1 - Initial velocities of hSULT1E1-catalyzed sulfation of estradiol (50 mu-M) with varied concentrations of PAPS

  Supplemental Figure 2 - Ki determinations for inhibitors of the hSULT1E1-catalyzed sulfation of estradiol

  Supplemental Figure 3 - Initial velocities of hSULT1A1*1-catalyzed sulfation of estradiol (5 mu-M) with varied concentrations of PAPS

  Supplemental Figure 4 - Ki determinations for inhibitors of the hSULT1A1*1-catalyzed sulfation of estradiol

  Supplemental Figure 5 - LC-MS analysis of endoxifen sulfate formed in an enzymatic reaction catalyzed by hSULT1E1

  Supplemental Figure 6 - LC-MS analysis of 4-TAM-SO4 formed in an enzymatic reaction catalyzed by hSULT1E1

  Supplemental Figure 7 - LC-MS analysis of N-desTAM-S formed in an enzymatic reaction catalyzed by hSULT1E1

  Supplemental Figure 8 - LC-MS analysis of 4-TAM-SO4 formed in an enzymatic reaction catalyzed by hSULT1A1*1

  Supplemental Figure 9 - LC-MS analysis of endoxifen-sulfate formed in an enzymatic reaction catalyzed by hSULT1A1*1

  Supplemental Figure 10 - LC-MS analysis of N-desTAM formed in an enzymatic reaction catalyzed by hSULT1A1*1