Data Supplement
- Supplemental Data -
Supplementary Table 1 - A list of back-calculated Quality control (QC) samples for ELISA assay developed to quantify total trastuzumab in media and cell lysate matrix
Supplementary Table 2 - A list of back-calculated Quality control (QC) samples for LC-MS/MS assay developed to quantify MMAE in media and cell lysate matrix
Supplementary Table 3- Sensitivity Indices obtained after Global Sensitivity Analysis (GSA) performed using a) SOBOL and b) PRCC method
Supplementary Figure 1 - ELISA standard curves generated for quantification of total trastuzumab in (A) media and (B) cell lysate samples
Supplementary Figure 2 - LC-MS/MS standard curves generated for quantification of unconjugated MMAE in (A) cell lysate and (B) media
Supplementary Figure 3 - Model generated and observed media and intracellular PK profiles of different ADC analytes after continuous exposure (panels A and C) and 2h exposure (panels B and D) of 75 nM T-