Data Supplement
- Supplemental Data -
Supplemental Table S1. Table showing sex of individual liver donors.
Supplemental Table S2. Table showing transitions for heavy labeled and unlabeled surrogate peptides for METTL7A (TMT1A) and METTL7B (TMT1B).
Supplemental Figure S1. Basic local alignment results for METTL7A (TMT1A) compared with METTL7B (TMT1B).
Supplemental Figure S2. Activity assay monitoring the formation of S-methyl captopril +/- DMPG by recombinant purified NGST-METTL7A (N-GST-TMT1A).
Supplemental Figure S3. Representative chromatogram traces from a TSL methylation activity assay with recombinant purified active (A) and boiled (B) N-GST-METTL7A (N-GST-TMT1A).
Supplemental Figure S4. Cartoon representation of class I methyltransferases (A) as described by Schubert et al., 2003.
Supplemental Figure S5. The methylation of TSL at various concentrations of purified recombinant N-GST-METTL7A (NGST-TMT1A) over the course of a 1 hr, 30 min, or 15 min incubation plotted with respect to protein concentration (A) or time increments (B).
Supplemental Figure S6. The relative luminescence unit response from purified recombinant METTL7A incubated with various concentrations of 4-chlorothiophenol.
Supplemental Figure S7. METTL7B (TMT1B) siRNA sequence with region complementary to METTL7A (TMT1A) coding region of NCBI Reference Sequence: NM_014033.4 highlighted (A).