Improvement of protein expression profile in three-dimensional renal proximal tubular epithelial cell spheroids selected based on OAT1 gene expression: a potential in vitro tool for evaluating human renal proximal tubular toxicity and drug disposition

Data Supplement

• Supplemental Data -

  Supplemental Figure S1. mRNA expression of kidney marker genes in 2D RPTECs, 3D RPTEC spheroids, and human kidney cortices.

  Supplemental Figure S2. Heatmap of 4473 proteins (A), 1492 enzymes (B), 112 SLC transporters (C), and 20 ABC transporters in 2D RPTECs at day12, 3D RPTEC spheroids at day 12/20 and human cortices.

  Supplemental Figure S3. Effect of cefalotin and cisplatin on intracellular ATP levels (A) and lactate dehydrogenase release (B) in 3D RPTEC spheroids.

  Supplemental Table S1. MS condition and ion monitoring parameters of analytes.

  Supplemental Table S2. Proteins showing more similar expression to human kidney cortex by changing 2D culture to 3D spheroidal culture.

  Supplemental Table S3. Proteins showing more deviated expression from human kidney cortex by changing 2D culture to 3D spheroidal culture.

  Supplemental Table S4. Coefficient of variation of mRNA expression in 3D RPTEC spheroids from 3 different donors and human kidney cortex from 5 different donors.

  Supplemental Table S5. Protein abundance in total lysate, plasma membrane and PM/TL ratio of detected proteins.