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- TABLE 1
Kinetic parameters for the cosubstrate UDPGA in the glucuronidation of three OH-PCBs
Results are mean ± S.D.; n = 4.
OH-PCB Concentration Vmax (Apparent) Km (Apparent) μM nmol/min/mg μM Liver 4′-OH-CB35 500 0.87 ± 0.20 697 ± 246 4′-OH-CB72 500 0.60 ± 0.10 684 ± 323 Intestine 4′-OH-CB69 200 0.20 ± 0.11 27 ± 14 - TABLE 2
Kinetic parameters for the glucuronidation of 4-OH-BP and OH-PCBs
Results are mean ± S.D; n = 4.
Substrate Intestine Liver Vmax (Apparent) Km (Apparent) Efficiency Vmax (Apparent) Km (Apparent) Efficiency pmol/min/mg μM μl/min/mg protein pmol/min/mg μM μl/min/mg protein 4-OH-BP 43 ± 10 599 ± 110 0.07 ± 0.02 182 ± 78 502 ± 235 0.48 ± 0.36 4-OH-CB1 504 ± 126 157 ± 18 3.2 ± 0.6 812 ± 326 150 ± 60a 5.5 ± 0.6 4-OH-CB2 417 ± 57 572 ± 47 0.73 ± 0.11 2277 ± 849 583 ± 95 3.95 ± 1.34 4′-OH-CB3 399 ± 110 412 ± 54 1.0 ± 0.4 317 ± 90 237 ± 58 1.4 ± 0.5 4′-OH-CB9 410 ± 99 297 ± 55 1.4 ± 0.3 751 ± 390 626 ± 318 1.4 ± 0.8 4-OH-CB14 255 ± 59 387 ± 65 0.67 ± 0.19 2022 ± 936 614 ± 202 3.31 ± 0.86 4′-OH-CB18 693 ± 223 15 ± 1a 47 ± 18 1225 ± 699 57 ± 24 20.8 ± 4.0 4′-OH-CB35 784 ± 348 265 ± 85 2.91 ± 0.87 2838 ± 1456 455 ± 89a 6.67 ± 3.55 4-OH-CB39 220 ± 90 134 ± 36 1.61 ± 0.37 1716 ± 536 242 ± 76 7.42 ± 2.28 4′-OH-CB68 213 ± 91 119 ± 75 2.09 ± 0.93 N.D. N.D. N.D. 4′-OH-CB69 751 ± 253 42 ± 21 20.7 ± 7.2 2774 ± 1153 1,071 ± 410 2.81 ± 1.18 4′-OH-CB72 401 ± 236 183 ± 126 2.43 ± 0.33 N.D. N.D. N.D. 4′-OH-CB79 124 ± 36 87 ± 21 1.47 ± 0.46 869 ± 318 476 ± 201 1.91 ± 0.50 4′-OH-CB106 431 ± 60 183 ± 58 2.52 ± 0.75 1579 ± 645 798 ± 122 2.00 ± 0.79 4′-OH-CB112 401 ± 67 163 ± 24a 2.52 ± 0.62 2144 ± 1007 1,643 ± 545 1.40 ± 0.63 4′-OH-CB121 220 ± 39 130 ± 21 1.70 ± 0.24 1046 ± 408 207 ± 97 5.27 ± 1.32 4′-OH-CB159 188 ± 66 213 ± 136 1.07 ± 0.46 681 ± 141 318 ± 91 2.26 ± 0.63 4′-OH-CB165 163 ± 26 137 ± 44 1.25 ± 0.30 404 ± 116 111 ± 28 3.77 ± 1.07 N.D., not done.
↵a S50 value given in place of Km because of atypical kinetics.
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