Metabolic disposition of triazolam and clobazam in humanized CYP3A mice with a double knockout background of mouse Cyp2c and Cyp3a genes

Data Supplement

• Supplemental Figure 1 -

  Supplemental Figure 1. Plasma concentrations of triazolam, 1’-hydroxytriazolam and 4hydroxytriazolam in WT (A), 3aKO (B) and hCYP3A/3aKO (C) mice.
  

• Supplemental Figure 2 -

  Supplemental Figure 2. Retention of CYP3A-MAC in hCYP3A/2c3aKO mice.
  

• Supplemental Figure 3 -

  Supplemental Figure 3. Expression CYP3A protein in the liver and intestine of hCYP3A/3aKO and hCYP3A/2c3aKO mice.
  

• Supplemental Figure 4 -

  Supplemental Figure 4. AUC ratios (metabolite/TRZ) in 2c3aKO, hCYP3A/2c3aKO, 3aKO and hCYP3A/3aKO mice.
  

• Supplemental Table 1 -

  Supplemental Table 1. AUC ratios of triazolam and its metabolites in WT, 3aKO and hCYP3A/3aKO mice. 
  

• Supplemental Table 2 -

  Supplemental Table 2. Primer list for genomic PCR analysis. 

• Supplemental Table 3 -

  Supplemental Table 3. Conditions used for LC-MS/MS analysis. 

• Supplemental Table 4 -

  Supplemental Table 4. Expression levels of mRNAs in the liver and intestine of WT, 3aKO, 2c3aKO, hCYP3A/3aKO and hCYP3A/2c3aKO mice.