Activation of Cryptic Donor Splice Sites Within the UGT1A First-Exon Region Generates Variant Transcripts That Encode UGT1A Proteins With Truncated Aglycone-binding Domains

Supplemental Fig. 1. Sequences of variant UGT1A first exons.

Supplemental Fig. 2. Predicted sequences for variant UGT1A proteins.

Supplemental Fig. 3. Sequence reads for variant UGT1A transcripts.

Supplemental Fig. 4. Expression of variant transcripts 1A8_n2 and 1A9_n2 in normal and drug-metabolizing tissues.

Supplemental Fig 5. Variant UGT1A transcripts and proteins.

Supplemental Fig. 6. Sequencing results identified the 1A8_n2 transcript in HT-29 cells.

Supplemental Fig. 7. Sequencing results identified the 1A10_n7 transcript in HT-29 cells.

Supplemental Fig. 8. Variant transcripts UGT1A8_n2 and UGT1A10_n7 generated using novel cryptic donor splice sites within UGT1A first exons in colorectal cancer HT-29 cells.

Supplemental Table 7: Known UGT1A transcripts and novel UGT1A variant transcripts identified in this study that are named using the current UGT1A nomenclature.

Supplemental Table 1: The number of Seqeuce reads identified from the RNA-seq datasets of the following study that have UGT1A exon 2 ligated to any sequences within the UGt1A first-exon region.



Supplemental Table 2: The number of Seqeuce reads identified from the RNA-seq datasets from the following study for all 24 variant transcripts.



Supplemental Table 3: The number of Seqeuce reads identified from the RNA-seq datasets from the following study for UGT1A variant transcripts as indicated.



Supplemental Table 4: The number of Seqeuce reads identified from the RNA-seq datasets from the following study for UGT1A variant transcripts as indicated.



Supplemental Table 5: The number of Seqeuce reads identified from the RNA-seq datasets from the following study for UGT1A variant transcripts as indicated.



Supplemental Table 6: The number of Seqeuce reads identified from the RNA-seq datasets from the following study for UGT1A variant transcripts as indicated.