Abstract
Distribution of microsomal glutathione transferase (mGST) protein in rat tissues was investigated by immunohistochemistry. Studies on the localization of mGST are of interest because of its involvement in the detoxication and bioactivation of xenobiotics. mGST antigen was detected in the cytoplasm of some hepatocytes and in bile ducts. In kidney, focal staining of mGST was observed in distal tubules and collecting ducts. Cerebral cortical and cerebellar Purkinje neurons showed good immunoreactivity, and nuclear staining was observed in the choroid plexus. The antigen was detected in epithelial cells of respiratory bronchioles and in the crypt cells of the duodenum. Exocrine cells of the pancreas stained for mGST. Nuclear immunostaining for this protein was observed in primary spermatocytes. mGST antigen was detected in the cytoplasm of the adrenal medulla as a granular stain. Leydig and Sertoli cells in testis also stained for the antigen. Distribution of mGST protein differs from that observed with cytosolic transferases and may be important in determining cell-selective susceptibility to xenobiotics.
Footnotes
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Send reprint requests to: Dr. M. W. Anders, Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Avenue, Box 711, Rochester, NY 14642.
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This study was supported by the National Institute of Environmental Health Sciences Grant ES03127 (to M.W.A.).
- Abbreviations used are::
- GST
- glutathione transferase
- mGST
- microsomal glutathione transferase
- PBS
- phosphate-buffered saline
- Received June 21, 1996.
- Accepted September 27, 1996.
- The American Society for Pharmacology and Experimental Therapeutics
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