Abstract
The aim of this study was to determine the selectivities of chemical inhibitors for human cytochrome P450 (P450) isoforms toward the corresponding rat P450 isoforms by using cDNA-expressed rat P450s (CYP1A2, CYP2A1, CYP2C6, CYP2C11, CYP2D2, CYP2E1, CYP3A1, and CYP3A2). Among the inhibitor probes for human P450s used in this study, only sulfaphenazole showed a selective inhibitory effect on the activity of the corresponding rat P450 isoform (CYP2C6). Furafylline also preferentially inhibited the activity of rat CYP1A2. However, methoxalen and ketoconazole more strongly inhibited the activities of other P450 isoforms than those of the corresponding rat P450 isoforms, CYP2A1 and CYP3A1/2, respectively. On the other hand, quinidine and aniline had little effect on the activities of the corresponding rat P450 isoforms, CYP2D2, and rat CYP2E1, respectively. These results suggest that chemical probes that have been used for human P450 isoforms do not always exhibit the same selectivity for the corresponding rat P450 isoforms. However, it appears that sulfaphenazole can be used as a selective inhibitor for rat CYP2C6. In addition, furafylline may also be a relatively selective inhibitor for rat CYP1A2.
Footnotes
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↵1 Abbreviations used are: P450, cytochrome P450; POD, phenacetin O-deethylation; T7H, testosterone 7α-hydroxylation; DFH, diclofenac 4-hydroxylation; T16H, testosterone 16α-hydroxylation; BLH, bufuralol 1′-hydroxylation; PNPH, p-nitrophenol 2-hydroxylation; MD4H, midazolam 4-hydroxylation; HPLC, high-performance liquid chromotography.
- Received February 4, 2003.
- Accepted March 19, 2003.
- The American Society for Pharmacology and Experimental Therapeutics
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