Abstract
β-glucuronidase cleavage of 4-methylumbelliferyl β-d-glucuronide generates the highly fluorescent compound, 4-methylumbelliferone. We show that other β-d-glucuronide compounds act as competitors in this assay. The 4-methylumbelliferyl β-d-glucuronide cleavage assay can easily be adapted to high throughput formats to detect the presence of β-D glucuronides generated using recombinant glycosyl transferase preparations.
Footnotes
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Send reprint requests to: Peter M. Shaw, Bristol-Myers Squibb, New Jersey, P.O. Box 5400, Princeton, NJ 08543-5300. E- mail:shawp{at}bms.com; and Olga V. Trubetskoy, PanVera Corporation, 545 Science Dr., Madison, WI 53711. E-mail: olgat{at}panvera.com
- Abbreviations used are::
- UDPGA
- uridine 5′-diphosphoglucuronic acid, UGT
- UDP-glycosyltransferase.
- Received September 3, 1998.
- Accepted January 29, 1999.
- The American Society for Pharmacology and Experimental Therapeutics
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A Fluorescent Assay Amenable to Measuring Production of β-d-Glucuronides Produced from Recombinant UDP-Glycosyl Transferase Enzymes
