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Rapid CommunicationShort Communication

A Fluorescent Assay Amenable to Measuring Production of β-d-Glucuronides Produced from Recombinant UDP-Glycosyl Transferase Enzymes

Olga V. Trubetskoy and Peter M. Shaw
Drug Metabolism and Disposition May 1999, 27 (5) 555-557;
Olga V. Trubetskoy
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Peter M. Shaw
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Abstract

β-glucuronidase cleavage of 4-methylumbelliferyl β-d-glucuronide generates the highly fluorescent compound, 4-methylumbelliferone. We show that other β-d-glucuronide compounds act as competitors in this assay. The 4-methylumbelliferyl β-d-glucuronide cleavage assay can easily be adapted to high throughput formats to detect the presence of β-D glucuronides generated using recombinant glycosyl transferase preparations.

Footnotes

  • Send reprint requests to: Peter M. Shaw, Bristol-Myers Squibb, New Jersey, P.O. Box 5400, Princeton, NJ 08543-5300. E- mail:shawp{at}bms.com; and Olga V. Trubetskoy, PanVera Corporation, 545 Science Dr., Madison, WI 53711. E-mail: olgat{at}panvera.com

  • Abbreviations used are::
    UDPGA
    uridine 5′-diphosphoglucuronic acid, UGT
    UDP-glycosyltransferase.
    • Received September 3, 1998.
    • Accepted January 29, 1999.
  • The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 27 (5)
Drug Metabolism and Disposition
Vol. 27, Issue 5
1 May 1999
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Rapid CommunicationShort Communication

A Fluorescent Assay Amenable to Measuring Production of β-d-Glucuronides Produced from Recombinant UDP-Glycosyl Transferase Enzymes

Olga V. Trubetskoy and Peter M. Shaw
Drug Metabolism and Disposition May 1, 1999, 27 (5) 555-557;

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Rapid CommunicationShort Communication

A Fluorescent Assay Amenable to Measuring Production of β-d-Glucuronides Produced from Recombinant UDP-Glycosyl Transferase Enzymes

Olga V. Trubetskoy and Peter M. Shaw
Drug Metabolism and Disposition May 1, 1999, 27 (5) 555-557;
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